Enzymes that survive the process conditions, not the lab bench.
Industrial bioprocesses run at temperatures, pH ranges, and solvent concentrations that no wild-type enzyme was evolved for. ProtSynq scores stability mutations under your target process conditions before any bioreactor run.
Thermostability engineering for process conditions
Process conditions in
Specify your target conditions: operating temperature, pH range, and any solvent or cofactor requirements. ProtSynq adjusts the scoring function to weight thermostability and pH-relevant electrostatics accordingly.
Stability scan
All single-residue substitutions across the full protein are scored for thermostability uplift. Surface salt bridges, hydrophobic core packing, and loop entropy contributions are explicitly modeled. Disulfide engineering suggestions are flagged separately.
Shortlist + structure output
Output: top-5 thermostabilizing variants with predicted ΔTm, the per-residue heatmap, and downloadable PDB structures. Secondary: positions tolerant to combinatorial mutations, for teams that want to build multi-substitution variants.
Why upstream computational screening pays off
Performance metrics from internal evaluation on thermostability benchmarks for industrial enzyme families (glycoside hydrolases, lipases, proteases).
Tell us your process conditions.
Industrial applications with unusual process requirements (high temperature, extreme pH, solvent tolerance) are a strong fit for the Institutional tier. Contact us to scope the workflow.