From 10,000 candidates to five worth testing.
Enzyme engineering campaigns stall not because good mutations don't exist — they stall because screening every candidate is expensive and slow. ProtSynq collapses the variant space before you open a 96-well plate.
The enzyme engineering workflow with ProtSynq
Structure in
Upload your enzyme PDB or AlphaFold model. ProtSynq identifies the active site, the substrate-binding pocket, and all solvent-exposed positions that could affect thermostability without disrupting catalysis.
Objective defined
Set your objective: thermostability uplift (ΔΔG stability negative), preserved catalytic activity (active site substitutions flagged), or both under a Pareto constraint. Specify any positions you want to exclude.
Variants ranked
ProtSynq scores all single-residue substitutions and returns the top-5 ranked by predicted Tm uplift and confidence. You get the shortlist, the per-residue heatmap, and downloadable PDB diffs for each candidate.
What changes when you run the computation first
Internal evaluation against published enzyme engineering benchmarks. These reflect ProtSynq's performance on representative cases, not cherry-picked best-case outcomes.
Run it on your enzyme.
The Explore tier is free. Upload your structure, define your thermostability objective, and see the top-5 output — before committing any wet-lab time.