Enzyme Engineering
Skip the combinatorial library. Score all single substitutions and select the five most likely to improve catalytic stability.
Learn more
Computational Protein Engineering
Predict which mutations fold and bind.
ProtSynq scores every point mutation for ΔΔG stability and ΔΔG binding — so enzyme and antibody engineers start wet lab with five ranked variants, not five hundred.
~3 months
typical wet-lab screening cycle for a single enzyme variant campaign
< 4 hrs
to score all single-residue substitutions across a 350-residue protein with ProtSynq
5 variants
high-confidence candidates delivered — not a ranked list of thousands
From structure to shortlist in four steps.
01
Upload your structure
PDB file or AlphaFold model. ProtSynq parses chain topology and identifies all mutable positions.
02
Set your objective
Maximize thermostability, tune binding affinity for a target chain, or optimize both under a Pareto constraint.
03
Run the scan
ProtSynq evaluates every single-residue substitution using an ensemble of physics-informed and learned scoring functions.
04
Get your ranked shortlist
Five variants ranked by predicted stability and affinity, with per-residue confidence scores and downloadable PDB diffs.
Your output: ranked, downloadable, actionable.
ProtSynq delivers a ranked variant table with per-mutation thermodynamic scores and one-click PDB exports.
ProtSynq — variant_output_LacZ_Q35M_campaign.csv
| Rank | Variant ID | Mutation | ΔΔG Stability (kcal/mol) | ΔΔG Binding (kcal/mol) | Confidence | Export |
|---|---|---|---|---|---|---|
| #1 | PSQ-LZ-0042 | A123V | −1.84 | −0.62 | 0.91 | |
| #2 | PSQ-LZ-0017 | L87I | −1.51 | −0.38 | 0.88 | |
| #3 | PSQ-LZ-0091 | T204A | −1.29 | +0.11 | 0.83 | |
| #4 | PSQ-LZ-0055 | V316L | −0.97 | −0.44 | 0.76 | |
| #5 | PSQ-LZ-0138 | I251V | −0.82 | +0.07 | 0.72 |
Built for the engineers who can't afford to screen everything.
Antibody Optimization
Identify CDR mutations that improve affinity without destabilizing the VH/VL fold. Filter by predicted Tm shift.
Learn moreIndustrial Biotech
Engineer thermostable biocatalysts for high-temperature process conditions — guided by predicted Tm uplift, not trial and error.
Learn moreCambridge, MA — Kendall SquareEmbedded in the biotech and life sciences ecosystem we serve.
Independent research team, founded 2022Science-first, no enterprise overhead — direct access to the founders.
Curated thermodynamic training dataTrained on ProThermDB and ProtaBank — not AlphaFold priors alone.
Direct scientific contact[email protected]
Ready to reduce your wet-lab screening cycle?
Request access to ProtSynq for your next enzyme or antibody campaign.